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KMID : 0613820030130030241
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Journal of Life Science
2003 Volume.13 No. 3 p.241 ~ p.247
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Enhanced Sensitivity and Long-Term G2/M Arrest in Adriamycin-treated DNA-PK-null Cells are Unrelated to DNA Repair Defects
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Kim Chung-Hui
Kim Jong-Shu
Han Jin
Kim Eui-Yong
Kim Na-Ri
Dang Van Cuong
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Abstract
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While the DNA-protein kinase (DNA-PK) complex, comprised of DNA-PKcs and Ku80, is primary involved in the repair of DNA double-strand breaks, it is also believed to participate in additional cellular processes. Here, treatment of embryo fibroblasts (MEFs) derived from either wild-type (Wt) or DNA-PKcs-null (DNA-PKcs-/-) mice with various stress inducing agents revealed that adriamycin was markedly more cytotoxic for Ku80-/-MEFs and led to their long-term accumulation in the G₂/M phase. This differential response was not due to differences in DNA repair, since adrimycin-triggered DNA damage was repaired with comparable efficiency in both Wt and Ku80-/-MEFs, but was associated with differences in the expression of important cell cycle regulatory genes. Our results support the notion that Ku80-mediated cytoprotection and G₂/M-progression are not only dependent on the cell’s DNA repair but also may reflect Ku80’s influence on additional cellular processes such as gene expression.
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KEYWORD
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DNA-protein kinase, Ku80, adriamycin, DNA repair
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